Below is the list of custom synthesized peptides by Celtek Biosciences and Biomatik that we used for synthesizing [4Fe-4S]-maquettes and then studying their redox properties and biomimetic activity.
Bioinspired Radical SAM Peptides
| Mw | = | 1010.16 g mol-1 | pI | = | 5.24 |
| εred. | = | 1490 M-1 cm-1 | instability index | = | 70.73 |
| charge | = | -2 (at pH 8) | more info … | ||
| Mw | = | 960.10 g mol-1 | pI | = | 4.37 |
| εred. | = | 1490 M-1 cm-1 | instability index | = | 78.62 |
| charge | = | -1 (at pH 8) | more info … | ||
| Mw | = | 961.14 g mol-1 | pI | = | 7.96 |
| εred. | = | 1490 M-1 cm-1 | instability index | = | 32.83 |
| charge | = | neutral (at pH 8) | more info … | ||
Nascent Radical SAM Maquettes
| Mw | = | 695.85 g mol-1 | pI | = | 5.5 |
| εred. | = | est. M-1 cm-1 | instability index | = | 53.06 |
| charge | = | -1 (at pH 8) | more info … | ||
| Mw | = | 612.69 g mol-1 | pI | = | 5.5 |
| εred. | = | est. M-1 cm-1 | instability index | = | 55.02 |
| charge | = | -1 (at pH 8) | more info … | ||
| Mw | = | 860.03 g mol-1 | pI | = | 5.50 |
| εred. | = | 1490 M-1 cm-1 | instability index | = | 48.28 |
| charge | = | -1 (at pH 8) | more info … | ||
| Mw | = | 775.87 g mol-1 | pI | = | 5.50 |
| εred. | = | 1490 M-1 cm-1 | instability index | = | 50.02 |
| charge | = | -1 (at pH 8) | more info … | ||
| Mw | = | 718.82 g mol-1 | pI | = | 5.50 |
| εred. | = | 1490 M-1 cm-1 | instability index | = | 28.99 |
| charge | = | -1 (at pH 8) | more info … | ||
Nascent Ferredoxin Peptides
| Mw | = | 639.80 g mol-1 | pI | = | 5.50 |
| εred. | = | est. M-1 cm-1 | instability index | = | 121.99 |
| charge | = | -1 (at pH 8) | more info … | ||
| Mw | = | 555.64 g mol-1 | pI | = | 5.50 |
| εred. | = | est. M-1 cm-1 | instability index | = | 43.83 |
| charge | = | -1 (at pH 8) | more info … | ||
| Mw | = | 802.99 g mol-1 | pI | = | 5.50 |
| εred. | = | 1490 M-1 cm-1 | instability index | = | 107.99 |
| charge | = | -1 (at pH 8) | more info … | ||
| Mw | = | 718.83 g mol-1 | pI | = | 5.50 |
| εred. | = | 1490 M-1 cm-1 | instability index | = | 39.6 |
| charge | = | -1 (at pH 8) | more info … | ||
Ferredoxin Control Peptides
| Mw | = | 1527.81 g mol-1 | pI | = | 5.97 |
| εred. | = | 5500 M-1 cm-1 | instability index | = | 76.83 |
| charge | = | -1 (at pH 8) | more info … | ||
| Mw | = | 1495.75 g mol-1 | pI | = | 5.98 |
| εred. | = | 5500 M-1 cm-1 | instability index | = | 76.83 |
| charge | = | -1 (at pH 8) | more info … | ||
Note 1: Isoelectric point (pI) corresponds to a pH value at which net charge of protein is zero.
Note 2: Given that the pKa of the N-terminal is expected to be in the range of 6.8 and 9.1 [10.1038/srep43748], we considered the N-terminal to be non-protonated at pH = 8.0.
Note 3: Molar extinction coefficients (ε) for peptides without aromatic residues were estimated from the additivity of peptide carbonyl charge transfer bands at λ = 205 nm [PubMed 14907727, 10.1002/pro.2253].
Note 4: The instability index provides an estimate of the stability of your protein in a test tube [10.1093/protein/4.2.155]. A protein with instability index that is smaller than 40, is predicted as stable; a value above 40 predicts that the protein may be unstable.
Iron-Sulfur Maquette Chemistry 